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dc.contributor.authorDowling, Paul
dc.contributor.authorHayes, Catriona
dc.contributor.authorTing, Kay R
dc.contributor.authorHameed, Abdul
dc.contributor.authorMeiller, Justine
dc.contributor.authorMitsiades, Constantine
dc.contributor.authorAnderson, Kenneth C
dc.contributor.authorClynes, Martin
dc.contributor.authorClarke, Colin
dc.contributor.authorRichardson, Paul
dc.contributor.authorO’Gorman, Peter
dc.date.accessioned2014-11-04T10:15:30Z
dc.date.available2014-11-04T10:15:30Z
dc.date.issued2014-10-17
dc.identifier.citationBMC Genomics. 2014 Oct 17;15(1):904en_GB
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2164-15-904
dc.identifier.urihttp://hdl.handle.net/10147/333615
dc.description.abstractAbstract Background Bone destruction is a feature of multiple myeloma, characterised by osteolytic bone destruction due to increased osteoclast activity and suppressed or absent osteoblast activity. Almost all multiple myeloma patients develop osteolytic bone lesions associated with severe and debilitating bone pain, pathologic fractures, hypercalcemia, and spinal cord compression, as well as increased mortality. Biomarkers of bone remodelling are used to identify disease characteristics that can help select the optimal management of patients. However, more accurate biomarkers are needed to effectively mirror the dynamics of bone disease activity. Results A label-free mass spectrometry-based strategy was employed for discovery phase analysis of fractionated patient serum samples associated with no or high bone disease. A number of proteins were identified which were statistically significantly correlated with bone disease, including enzymes, extracellular matrix glycoproteins, and components of the complement system. Conclusions Enzyme-linked immunosorbent assay of complement C4 and serum paraoxonase/arylesterase 1 indicated that these proteins were associated with high bone disease in a larger independent cohort of patient samples. These biomolecules may therefore be clinically useful in assessing the extent of bone disease.
dc.language.isoenen
dc.subjectBONE DENSITYen_GB
dc.subject.otherBONE DISEASEen_GB
dc.subject.otherGENOMICSen_GB
dc.titleIdentification of proteins found to be significantly altered when comparing the serum proteome from Multiple Myeloma patients with varying degrees of bone diseaseen_GB
dc.typeArticleen
dc.language.rfc3066en
dc.rights.holderPaul Dowling et al.; licensee BioMed Central Ltd.
dc.description.statusPeer Reviewed
dc.date.updated2014-10-27T12:03:47Z
refterms.dateFOA2018-08-08T14:11:28Z
html.description.abstractAbstract Background Bone destruction is a feature of multiple myeloma, characterised by osteolytic bone destruction due to increased osteoclast activity and suppressed or absent osteoblast activity. Almost all multiple myeloma patients develop osteolytic bone lesions associated with severe and debilitating bone pain, pathologic fractures, hypercalcemia, and spinal cord compression, as well as increased mortality. Biomarkers of bone remodelling are used to identify disease characteristics that can help select the optimal management of patients. However, more accurate biomarkers are needed to effectively mirror the dynamics of bone disease activity. Results A label-free mass spectrometry-based strategy was employed for discovery phase analysis of fractionated patient serum samples associated with no or high bone disease. A number of proteins were identified which were statistically significantly correlated with bone disease, including enzymes, extracellular matrix glycoproteins, and components of the complement system. Conclusions Enzyme-linked immunosorbent assay of complement C4 and serum paraoxonase/arylesterase 1 indicated that these proteins were associated with high bone disease in a larger independent cohort of patient samples. These biomolecules may therefore be clinically useful in assessing the extent of bone disease.


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